It focuses on a young white mouse named Dmitri who's wants to have an adventure and wants to be independent who don't wants his owner to help him whenever he goes until he meets a red basset hound named Lisa who is the pet of the owner's best friend. at first they don't like each other, until they start to like each other right after Lisa saved Dmitri from a cat who tried to eat him until then they start to have a chemistry between the two, together they go on this long journey of life.
Dmitri- a young white mouse who is the pet of a girl named Abigail, Dmitri is sweet, kind, innocent, naive brave, heroic, free-sprited young mouse who wants to be free and see the world on his own. his life fully change when meets Lisa. throughout the film, he has to remind Lisa that he's not a lab rat.
Lisa- a red basset hound who is the pet of a girl named Clara who lived across the street from Clara, Lisa is funny, strong, comedic, optimistic, loyal, friendly, protective, confident, nice who also wants to have an adventure she may have a big belly but she has a big heart and really smart, who becomes Dmitri's best friend and voice of reason. she calls Dmitri a “lab rat” because he reminds her of one.
Abigail - a blond haired tomboy girl who is the owner of Dmitri who loves him very much and never sees him as some dumb rat, she also has two brothers named Richard and Seth. it revealed later in the film is that Clara is her best friend.
Clara- a brown haired girl who is the fiend of Abigail and owner of Lisa. she's smart, kind, witty, sweet, and optimistic as her pet Lisa.
Sam - a friend of Clara and Abigail.
Brian - a nerdy boy who is the friends of Sam, Clara and Abigail. Brian has a crush on Abigail who tries to tells her that she likes her so much.
Oz- a big nerdy boy and twin brother of Brian, who's also a friend of Clara and is a video game enthusiastic.
Mackey - a black dog-rabbit who is the annoying friend of Lisa and usually serves as comic relief.
Skippy - a tall dog who is very nerdy and shy who is a friend of Lisa who has a huge crush on her.
Bernard- a big dog who is the serious and very wise friend of Lisa.
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